P-153. The isolation and culture of human primordial follicles from fresh ovarian tissue
نویسندگان
چکیده
منابع مشابه
Depletion of Ovarian Primordial Follicles after Gamma Irradiation
Introduction:Artifical sourace of radiation have increased worldwide average annual exposure of mankind and could especially affect rapidly dividing cells in gonads. Since, radiation sensitivities of different components of primordial follicles, and the time required for their deplation have not been morphologically eatablished we decided to estimate the time required for depletion of primordi...
متن کاملIn vitro development of primordial follicles after long-term culture of goat ovarian tissue.
This study aims to investigate the effects of follicle stimulating hormone (FSH) and fibroblast growth factor-2 (FGF-2) on the survival and growth of caprine preantral follicles. Ovarian tissues were cultured for 1, 7, 14, 21 or 28 days in medium supplemented with FSH (FSH-2d or FSH-7d, i.e., with replacement of the culture medium every 2 or 7 days, respectively) or FSH+FGF-2 (replacement of th...
متن کاملP-219: GDF9, BMP15 and Their Receptors Expression During In Vitro Culture of Mouse Vitrified Ovarian Tissue Derived Preantral Follicles
Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...
متن کاملP9: Isolation and Culture of Adult Microglia Cells Derived from Epileptic Human Brain Tissue
لطفاً به چکیده انگلیسی مراجعه شود.
متن کاملViability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue.
OBJECTIVE To develop an efficient technique to preserve primordial follicles from cryopreserved ovarian tissue. DESIGN Frozen-thawed and fresh preantral follicles were mechanically isolated for viability testing, and their morphology was histologically analyzed. SETTING Laboratory of Animal Reproduction, Faculty of Veterinary Medicine and Zootechny, University State of São Paulo, Brazil. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Human Reproduction
سال: 1997
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/12.suppl_2.192-a